In addition, the invasion (Fig. 2I, J) and migration (Figure S2C, D) capability test using the transwell assay showed that the loss of Circ-RAPGEF5 significantly impaired the migration and invasion of ICC cells, while overexpression of Circ-RAPGEF5 enhanced this ability. The gene discussed is RAPGEF5; the disease is intrahepatic cholangiocarcinoma.