As expected, R848@M2pep-MPsAFP or anti-PD-1 antibody alone significantly inhibited tumor growth, with 56.6% or 36.7% reduction in tumor weight (Fig. 7b, c), and 50.9% or 46.0% reduction in tumor nodule numbers (Fig. 7b, d), respectively. The gene discussed is PDCD1; the disease is neoplasm.