In view that R848@M2pep-MPsAFP-reprogrammed M2-like macrophages efficiently activated CD8+ T cells, to investigate that the enhanced anticancer activity and antitumor immune response were mediated by R848@M2pep-MPsAFP-reprogrammed macrophages, clodronate liposomes were applied to deplete macrophages in the orthotopic Hepa1-6 tumor-bearing mice50. Here, CD8A is linked to neoplasm.