Flow cytometric analysis showed that R848@M2pep-MPsAFP significantly increased the numbers of M1-like TAMs including CD80+ TAMs (Fig. 4b), CD86+ TAMs (Fig. 4c) and MHC II+ TAMs (Fig. 4d), while decreasing the numbers of M2-like TAMs, such as CD206+ TAMs (Fig. 4e) in tumor tissues, validating that R848@M2pep-MPsAFP efficiently reprogrammed M2-like TAMs into M1-like phenotype. The gene discussed is CD80; the disease is neoplasm.