We next used BONCAT to isolate the individual nascent proteomes followed by LC-MS/MS from AHA-labelled whole hippocampus (tg37+/− mice) and ANL-labelled hippocampal neurons (NCAT::CamK2a mice) and astrocytes (NCAT::GFAP mice) for each of the three experimental conditions (NBH control, prion-diseased, prion-disease + trazodone treatment) (see schematic in Fig. 2A). Here, GFAP is linked to prion disease.