In survival analysis of low-TMB group, GC patients with lower expression of GPR27 displayed longer OS (HR=1.34, 95%CI:0.85-2,13, P=0.21, Supplementary Figure 2C) and DFS (HR=2.52, 95%CI:1.02-6.21, P=0.037, Supplementary Figure 2D). The gene discussed is GPR27; the disease is gastric cancer.