Firstly, we determined the genes expression in PBMCs by qRT-PCR analysis, and found the expression of NSUN7, NOP2, PUS1 and PUS3 in septic patients (septic shock and sepsis) at day 1 were significantly higher than in healthy volunteers, and FTO expression was lower in septic patients (Figure 10A). Here, PUS3 is linked to septic shock.