S100A8 overexpression in cholangiocarcinoma could facilitate in vitro and in vivo cell invasion and migration by activating the NF-κB pathway as well as increasing VEGF expression, while S100A8 knockdown or TLR4 and NF-κB inhibition could significantly inhibit cell migration and metastasis (Pan et al., 2020). This evidence concerns the gene NFKB1 and cholangiocarcinoma.