The focus of this study is to evaluate the T-regs in the PE group compared to the NP group and NW group and to correlate the T-regs with inflammatory markers like TGF-β1, IL-6, and hs-CRP in the pathophysiology and for early diagnosis of preeclampsia.A total of 50 subjects were recruited for this study, out of which 20 subjects were PE cases, 20 were normal pregnant women, and 10 were normal non-pregnant reproductive age group women. The gene discussed is TGFB1; the disease is preeclampsia.