In a previous in silico study carried out by Belal et al. [19], it was found that benzopyran-4-one moiety, a constituent of C. officinalis, is potent against matrix metalloproteinase 8 and matrix metalloproteinase 9 (MMP 8 and MMP 9) and hence it was suggested that Calendula can be used in the treatment of diabetic foot ulcers. This evidence concerns the gene MMP9 and diabetic foot.