Next, by immunoblotting, we demonstrated thatFUNDC2 silencing in the triple-negative breast cancer cell line MDA-MB-231 can indeed reduce the expression of AKT and GSK3β phosphorylation (p-AKT and p-GSK3β) at the protein level, which was further validated in xenografted MDA-MB-231 cells in BALB/C-nude mouse tissue (Figures 5D and6E). Here, AKT1 is linked to triple-negative breast carcinoma.