Initially, we set out to determine the endogenous canonical Wnt signaling activities in different HCC cell lines, that is, HUH7, SNU475, Hep3B, HepG2, SNU398, and Mahlavu cells, all of which have mutations in at least one component (β‐CATENIN, APC, or AXIN‐1) of Wnt/β‐catenin signaling, along with the control HEK293T cells. Here, AXIN1 is linked to hepatocellular carcinoma.