In other words, the global influence of “cancer” on the membrane lipidome is greater than that of “Wnt pathway manipulation.” Second, according to the vertical clustering of the heatmap, we identified five main patterns of DRLs: (a) Lipids that were generally Up in HCC cells (with or without Wnt pathway manipulation) as compared to healthy control cells and weakly responsive (mostly as downregulation) to Wnt pathway manipulation, particularly to Wnt3a. This evidence concerns the gene WNT3A and hepatocellular carcinoma.