Previous work has shown that ancestral exposure to abnormal diets (such as HFD and HSD) led to various metabolic dysfunctions in the offspring, including cardiac lipotoxicity, diabetes, and obesity (Chen et al., 2022; Guida et al., 2019; Kaspar et al., 2020; Wan et al., 2022). This evidence concerns the gene CHST3 and obesity due to melanocortin 4 receptor deficiency.