Diabetes upregulated the expressions of GSDMD, caspase-1, NLRP3 and p62, and downregulated microtubule-associated protein 1 light chain 3B (LC3B) II/I ratio in the rat model of cerebral I/R injury with diabetes, which were abolished by hypothermia, suggesting that hypothermia increased autophagy and downregulated pyroptosis. The gene discussed is CASP1; the disease is diabetes mellitus.