Using the wound healing assay, 48 h after the addition of TGFβ, we began to observe higher reporter activity in epithelial carcinoma cells, both partially mesenchymal, H1299 and H2170 VRCs cells39(Fig. 3A,B), predominantly at the edge of the wound, as it is often reported48,49. The gene discussed is TGFB1; the disease is carcinoma.