GOLPH3 and osteosarcoma: Next, each of these constructs was stably integrated into the U-2 Osteosarcoma (U-2 OS) cell line expressing stdMCP-HaloTag (synonymized tandem MS2 coat protein fused with HaloTag) for RNA labeling63,64, and scFv-sfGFP (single-chain antibody fused with superfolder GFP) for protein visualization65.