Chromatin alterations can regulate gene expression and epigenetic changes in vivo, including cancer initiation and progression.32 The result of Karyotype analysis presented the proportion of aneuploidy of selected Cbx4−/−, KrasG12D cells (62%) was significantly higher than KrasG12D counterpart (32%), implying the unstable genomic condition after long-time culture with CBX4 knockout (Fig. 4c). This evidence concerns the gene CBX4 and cancer.