It has also been studied in a variety of cancer species and its effect on tumor production is tissue heterogeneous.15–21 As mere CBX4 deficient does not induce LUAD (Supplementary Fig. 1), we chose KrasG12D mouse model for this study, widely used in lung cancer studies, and Adeno-Cre could be delivered via nasal inhalation to initiate KrasG12D-dependent lung cancer.25 We crossed Cbx4L/L with LSL-KrasG12D mice to get Cbx4L/L, LSL-KrasG12D mice for subsequent experiments. Here, CBX4 is linked to lung cancer.