After xenotransplantation of HT29 and HCT116 cells into female athymic nude mice, the tumor weight of 10 mg/kg every 2 days in xanthohumol-treated mice was significantly reduced, and expression of Ki-67, p-AKT, and HK2 was downregulated, which was consistent with the results of in vitro experiment (Liu et al., 2019). Here, HK2 is linked to neoplasm.