Furthermore, we determined the precise location of lymphatic vessels in murine MVs, and these lymphatic vessels were aberrantly expanded in EC-<i>Foxc1/c2</i> mutant mitral valves.<h4>Conclusions</h4>Our results indicate that <i>Foxc1</i> and <i>Foxc2</i> are required for maintaining the integrity of the MV, including VEC junctions, ECM organization, and lymphatic vessels to prevent myxomatous mitral valve degeneration. Here, FOXC1 is linked to Myxomatous mitral valve degeneration.