Our study involved a more accurate determination of the 15 fractions of bile acids, revealing that in the analysis of the ratio of 15 bile acid metabolites to total bile acids, the percentages of LCA and CDCA to total bile acids were relatively higher, while the percentages of CA, DCA, GDCA, GLCA, TLCA and GCDCA to total bile acids were relatively lower in PCOS patients. Here, GNAT3 is linked to polycystic ovary syndrome.