SPP1 and colorectal carcinoma: As shown in Figure 5D, the TCGA CRC cohort analysis by the EPIC, TIMER, MCPCOUNTE, XCELL, CIBERSORT, CIBERSORT-AS, and QUANTISEQ algorithms showed that SPP1 expression is negatively correlated with the purity of CRC tissues, but positively correlated with the infiltrated levels of multiple types of macrophages.