PLNs from both TNF-Tg mice with Early and Advanced arthritis exhibited an increased number of total sinus spatial spots compared to WT (Figure 1R; WT 20.0 ± 8.0, Early 56.2 ± 37.6, Advanced 77.3 ± 32.0 total sinus spots), while Advanced PLNs showed a greater proportion of Sinus-Immuno spots compared to Early PLNs (Figure 1S; WT 64.6 ± 7.8, Early 43.1 ± 27.0, Advanced 71.6 ± 17.7% Sinus-Immuno spots). This evidence concerns the gene TNF and Arthritis.