Recent attempts to reveal the mechanisms responsible for lymphatic dysfunction and Bin cell accumulation and translocation in PLN sinuses during Early vs Advanced arthritis in TNF-Tg mice utilized various bulk-tissue approaches (i.e., bulk RNA sequencing, flow cytometry and ex vivo cultures), which lack the resolution to identify molecular changes related to arthritic severity at the single-cell level (4). This evidence concerns the gene TNF and Arthritis.