Although modulation of cardiac autonomic activity using acetylcholinesterase (AChE) inhibitors, including donepezil and pyridostigmine, has been demonstrated to exert cardioprotection in many heart diseases (Khuanjing et al. 2020a, 2021a, 2021b; Lu et al. 2018), the anti-cardiotoxic effects of donepezil against TIC remain largely unknown. This evidence concerns the gene ACHE and heart disorder.