Similar to shFBXO21 KD, Selonsertib treatment led to decreased proliferation, increased apoptosis, but did not demonstrate the same cell surface phenotype as shFBXO21 KD further supporting ASK1 is not likely a substrate of FBXO21 in the context of AML (Supplementary Fig. 2B, F). Here, MAP3K5 is linked to acute myeloid leukemia.