CD274 and experimental autoimmune encephalomyelitis: In order to investigate the spatiotemporal expression of PD-L1 by astrocytes in the context of autoimmune CNS inflammation, we induced experimental autoimmune encephalomyelitis (EAE) in wild-type (WT) C57Bl/6 mice by immunization with myelin oligodendrocyte glycoprotein 35-55 (MOG35–55) in complete Freund’s adjuvant (CFA) followed by pertussis toxin (PTx) injection.