To assess whether phosphorylation of p110α affects the CRC progression, we endogenously mutated Y317 or Y508 to F (phenylalanine) on PIK3CA locus using CRISPR/Cas9 mediated genome editing strategy in colon cancer cells (HCT116, DLD1, SW480, LoVo) (Fig. S1C and S1D). The gene discussed is PIK3CA; the disease is colonic neoplasm.