The findings indicate that acetate played a positive role in regulating adipocyte differentiation and lipid deposition by activating MAPKs and the mTOR signaling pathway (the expression up-regulation of genes such as PPAR-γ, C/EBP-α and SREBP-1, etc.)and inhibiting the AMPK signaling pathway (the expression down-regulation of genes such as HSL, CPT-1 and AMPK-α, etc.), which is of great significance for the treatment of obesity, insulin resistance and other related diseases. This evidence concerns the gene PPARG and obesity due to melanocortin 4 receptor deficiency.