PCSK1 and obesity due to melanocortin 4 receptor deficiency: As a result, increased body weight, accumulated epididymal fat, and enlarged adipocytes showed small differences among FNC-L and FDIO-L mice, as well as blood GLP1 level and Gcg and Pc3 mRNA levels (Figure 3B–G, and Supplementary Figure S4, respectively), indicating that the effect of the microbiota shaped by the high-fat diet on developing obesity-related symptoms was somewhat limited.