In the present study, we provided evidence, for the first time to our knowledge, for the potent pro-angiogenesis function of ALDH1A3 by using both indirect co-culture and direct co-culture of ALDH1A3-overexpressing GBM cells with ECs in vitro and by applying an angiogenesis model in vivo. Here, ALDH1A3 is linked to glioblastoma.