As depicted in Figure 8A, LINC01749, PCDH9-AS1, TMEM220-AS1, and KCNMA1-AS1 exhibited relatively lower expression levels in ESCC cell lines (KYSE-30, KYSE-150, KYSE-410, TE-1, and Eca-109) compared to Het-1A. The gene discussed is TMEM220; the disease is esophageal squamous cell carcinoma.