Regarding gene cooperation networks, Zhao et al., performed a CRISPR/Cas9 screening coupled with single-cell RNA sequencing in genetically engineered human mammary epithelial MCF10A cells (MCF10-PTEN-/-, MCF-PI3KCA, and MCF10A-MYC) to identify how different driver genes could cooperate and how the combination of inactivated tumour suppressor genes could have an effect on the oncogenic properties and the transcriptome of breast epithelial cells [29]. This evidence concerns the gene PTEN and neoplasm.