In the phase II Keynote224 trial of pembrolizumab monotherapy, the conventional positive cell rate of PD-L1 in tumor cells (tumor proportion score (TPS)) and the combined positive score (combined positive score (CPS)) were calculated by dividing the number of PD-L1 positive cells in tumor cells and immune cells by the total number of surviving tumor cells and multiplying by 100 [73]. The gene discussed is CD274; the disease is neoplasm.