The gene profile of the original tumor showed moderate similarities to the profiles of PDSO. This could be due to the presence of different types of normal cells in the original tumor or changes in the genetic characteristics of the organoid cells.The gene expressions were grouped into four clusters, and cluster C was found in both the original tumor and ODXs. In this cluster, 225 genes and 15 biological pathways were identified, including those involved in cancer-related proteoglycan, cell cycle regulation, and the P53 signaling pathway. This evidence concerns the gene TP53 and cancer.