In the current study, transcriptomic analysis identified the transforming growth factor-β (TGFβ) signaling pathway to be significantly upregulated in dasatinib-resistant pre-BCR+/E2A-PBX1+ ALL cells, which was further investigated by functional characterization studies in pre-BCR+/E2A-PBX1+ as well as BCP-ALL cells. This evidence concerns the gene OPN1SW and acute lymphoblastic leukemia.