Different members of the TGFβ and BMP subfamilies were found to be upregulated; however, knowing their role in cancer and cell proliferation regulation, we pursued further functional studies investigating the role of the TGFβ/SMAD signaling pathway in the preclinical model of dasatinib-resistance in pre-BCR+/E2A-PBX1+ ALL, as well as in several BCP-ALL cell lines with different cytogenetics. This evidence concerns the gene PBX1 and acute lymphoblastic leukemia.