Apart from few studies investigating the role of autophagic key players like protein 1 light chain 3 B (LC3B), sequestome 1 (p62) and pro-autophagic Beclin-1 in patients’ tissue by immunohistochemistry during cholangiocarcinogenesis [16] or in combined hepatocellular carcinoma (HCC)-CCA [17], studies determining the impact of autophagy in primary tumor tissue of CCA patients remain scarce so far. This evidence concerns the gene BECN1 and neoplasm.