A temperature-independent GO analysis of the enriched DEGs between the two donor groups, p5 and p20, reported KEGG pathways (Figure 6b) related to hypertrophic cardiomyopathy, dilated cardiomyopathy, adherens junction, arrhythmogenic right ventricular cardiomyopathy, focal adhesion, cardiac muscle contraction, adrenergic signalling in cardiomyocytes, PI3KT-Akt signaling pathway, ECM-receptor interaction, regulation of actin cytoskeleton, HIF-1 signaling pathway, FoxO signaling pathway, cellular senescence, TGF-beta signaling pathway (Figure 6b), and Leukocyte transendothelial migration. The gene discussed is AKT1; the disease is hypertrophic cardiomyopathy.