Routine diagnostic analyses by DMD MLPA, and muscle gene panels showed no (likely) pathogenic variants in the coding regions of DMD or any LGMD-associated gene (especially SGCA, SGCB, SGCD, SGCG, ANO5, CAPN3, DYSF, FKRP, TCAP, CAV3, PYGM, MYOT) in both index patients. The gene discussed is DYSF; the disease is limb-girdle muscular dystrophy.