SP1 and neoplasm: In contrast, the silencing of AFAP1-AS1 suppressed tumor growth (group vector + sh-AFAP1-AS1 vs. group vector), which could be reversed by overexpression of SP1 (group vector + sh-AFAP1-AS1 vs. group ov-SP1 + sh-AFAP1-AS1) (Figure 4A,B).