RPPH1 and neoplasm: The copy number alterations of the genes F5, RPPH1 and CYP2C8 identified by the qPCR-based method in the primary tumour sample were confirmed by next-generation sequencing, and the absolute copy numbers of both the target and the reference genes established by sequencing and qPCR-based methods were congruent with each other (for 10 genes, slope: 0.9498, r2 = 0.7524) (Figure 1e).