CD274 and hepatocellular carcinoma: As shown through the graphical method in Figure 4A, HCC cells were adapted with the nontoxic concentration of 2 μM of 5-AZA for 7 days, then plated together in a cell-to-cell contact with phorbol myristyl acetate (PMA)-activated Jurkat cells for 24 h in the presence of 0.1 μg of functional antibody against PD-L1.