DNMT1 and hepatocellular carcinoma: Interestingly, in line with data in the animal model, the downregulation of PD-L1 in HCC cells was accompanied by the decrease of DNMT1 mRNA, both for a concentration of 5 and 20 nM, until up to a 60% decrease in Huh7 cells (p < 0.01) (Figure 3D).