Notably, regarding the lncRNA-regulatory TPM1 mechanism, some scholars reported that the alternative splicing mechanism of endogenous TPM1 exon 2a or 2b was found to be different in ESCC cells than in non-cancerous cells, and they identified a previously unlabeled nuclear lncRNA TPM1-AS in human cancer cells (reverse transcribed from the fourth intron region of TPM1) and demonstrated that it is involved in alternative splicing and cell motility of TPM1 mRNA precursor. The gene discussed is TPM1; the disease is esophageal squamous cell carcinoma.