To shed light on the role of cellular steatosis for the expression of IL-32 and CCL20 in MASLD, we treated two hepatoma cell lines, HepG2 and Huh7, with palmitic acid (PA, C16:0), known to induce cellular lipid accumulation, lipo-apoptosis and lipo-toxicity [18]. Here, CCL20 is linked to metabolic dysfunction-associated steatotic liver disease.