Differential gene expression profiling was conducted using the nCounter PanCancer Pathways Panel, which includes 770 genes representing major canonical cancer pathways: Wnt, Hedgehog, apoptosis, cell cycle, RAS, PI3K, STAT, MAPK, Notch, TGF-β, chromatin modification, transcriptional regulation, and DNA damage control. The gene discussed is TGFB1; the disease is cancer.