MBD3 and acute lymphoblastic leukemia: The location of these sgRNA sequences in ARID4B and MBD3, respectively, are indicated in Figure 3D and Figure 4D. For all tested sgRNAs directed against either ARID4B or MBD3, the number of GFP+ leukemic cells was progressively and significantly reduced over time in KMT2A-r ALL cells line SEM, but not in KMT2A-w ALL cell line 697 (Figure 3F and Figure 4E).