In the case of ARID4B, all tested sgRNAs showed low KO scores ranging from 1% to 35% in the KMT2A-r ALL cell line SEM, with the commercially available sgRNA (i.e., ARID4B_IDT_1AA) producing the lowest score (Figure 3G). The gene discussed is ARID4B; the disease is acute lymphoblastic leukemia.