For all three genes, the z-scores were significantly lower in KMT2A-r ALL cell lines as compared with KMT2A-w BCP-ALL cell lines (Figure 2A,E and Figure S2A) as were the read counts for individual sgRNA sequences on day 21 (Figure 2B–D,F–H and Figure S2B–D). The gene discussed is OPN1SW; the disease is acute lymphoblastic leukemia.