Serological proteome analysis (SERPA) is another proteomic approach and screening autoantibodies as serum biomarkers of B-ALL using SERPA with combination of 2-DE, immunoblotting and MS revealed that α-enolase and VDAC1 autoantibodies were promising biomarkers for children with B-ALL. The gene discussed is VDAC1; the disease is acute lymphoblastic leukemia.