Within the whole CJD cohort, CSF t-tau, 14–3-3, and NfL were significantly associated with survival in both univariate (HR 1.73 [1.49–2.02], P < 0.001; HR 1.72 [1.46–2.02], P < 0.001; and HR 1.27 [1.09–1.49], P = 0.002, respectively) and multivariate Cox regression analyses, yielding a prognostic performance in the range of that of SNAP-25 (in case of t-tau and 14–3-3) and Ng (in case of NfL). This evidence concerns the gene NEFL and Creutzfeldt Jacob disease.