The proportion of CD4+CXCR5+Foxp3+Tfr cells in lymphocytes of control pregnant mice, GDM mice, and GDM mice treated with probiotics was (0.84 ± 0.06)%, (0.28 ± 0.04)% (p < 0.0001, comparison between control pregnant mice and GDM mice), and (0.76 ± 0.07)% (p = 0.0001, comparison between GDM mice and GDM mice treated with probiotics), respectively (Fig. 6C). This evidence concerns the gene CD4 and gestational diabetes.