Our study found that in non-cirrhotic patients with NAFLD, risk factors for the aggravation of liver steatosis included male, younger age, weight, BMI, waist circumference, history of hyperuricemia, FBG, UA, TG, ALT, AST, low AST/ALT ratio, GGT and LSM. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.