Surprisingly in B16F10 melanoma tumors, anti-OX40/anti-PD-L1 treatment alone, independently of the diet, increased the percentage of total T lymphocytes (CD45+CD3+) (Fig. 2D, Supplementary Fig. 2A) and T helper cells (CD45+CD3+CD4+) (Supplementary Fig. 3A), and promoted the activation of cytotoxic effector T cell (CD3+CD8+CD44+GzmB+) (Fig. 2E) while reducing the T reg cell population (CD3+CD4+CD25+) (Supplementary Fig. 3B). The gene discussed is CD4; the disease is melanoma.