Significant levels of IL-2, IFN-ɣ and TNF-α were detected in supernatants of CD4+ nfP2X7-M-CAR-T cells co-cultured with MDA-MB-231 and U87 cancer cell lines, but not when co-cultured with either SK-ND-Z or K562 cells (Fig. 1d). This evidence concerns the gene TNF and cancer.