We evaluated the changes in polypeptide hormone-encoding genes in the MX1-positive STB from ZIKV-infected hTSC-organoids, which showed similar changes to those in the maternal circulation or placenta from pregnant women with PE, including increased CGA, CGB family genes, CRH, GIP, INHA, INSL4, LEP, LHB and TAC3 and decreased ADM, GAL, ACTN1 and ACTN4, suggesting a possible cause of PE by ZIKV infection (Fig. 6e)57. Here, ACTN4 is linked to Zika virus infectious disease.