CD58 and cancer: To validate the regulatory role of CMTM6 in CD58 and PD-L1 expression, we conducted CMTM6 deletion and reconstitution in cell lines derived from multiple cancer types, including 8505C thyroid cancer cells (Figures 1H and S1C), A375 melanoma cells (Figures 1I and S1D), and RKO colorectal cancer cells (Figures S1E and S1F).